In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold

Due to the prevalence of cardiovascular diseases and their growing trend throughout the world, including Iran, efforts to treat these types of diseases have increased. There are lots of therapies for restoring cardiovascular function include organ transplants, reconstructive surgery, the use of mechanical or artificial devices, and the use of metabolic products. Although these methods are commonly used, they cause problems due to donor limitations, such as biocompatibility, infection, and tissue rejection by the patient. Meanwhile, vascular tissue engineering with the aim of building biocompatible and efficient vessels to replace lost vessels has created high hopes for the treatment of lesions. In this article, we used polyglycerol sebacate polymer (PGS) due to the properties such as high biocompatibility, good cell adhesion, controllable degradation rate and desirable mechanical properties, and combined it with polycaprolactone polymer (PCL) and gelatin to fabricate 3D scaffolds by electrospinning method. We also added vascular endothelial growth factor and then analysed the endothelial differentiation of mesenchymal stem cells. The expression of CD31 and VEGF-R2 genes has been measured by qPCR method, which revealed reasonable results.