Therapeutic Effects and Molecular Mechanisms of KD025 in Intraocular Pressure Regulation

KD025 demonstrates effective IOP-lowering capabilities in rodent models. Its mechanism likely involves modulating TM cell contractility (<i>via</i> α-SMA reduction) and inhibiting cell migration. The unexpected findings on fibronectin and F-actin suggest that selective ROCK2 inhibition by KD025 induces complex and distinct effects on TM extracellular matrix dynamics and cytoskeletal organization compared to non-selective ROCK inhibitors. These results highlight ROCK2 as a promising therapeutic target for glaucoma, though its distinct cellular actions warrant further investigation.