Preparing for successful protein crystallization experiments
Crystal-based structural methods, including X-ray crystallography, are frequently utilized for the determination of high-resolution structures of biomolecules. All crystal-based diffraction methods first require the preparation of biomolecular crystals, and careful sample preparation for crystallization experiments can increase the frequency of success. In this article, strategies to optimize factors that can impact crystallization are presented, from which buffers and reducing agents are most favorable to which crystallization techniques could be used.
Authors
- Sarah Bowman (ORCID: https://orcid.org/0000-0002-7426-7328)
- V. Stojanoff (ORCID: https://orcid.org/0000-0002-6650-512X)
- Gabrielle R. Budziszewski (ORCID: https://orcid.org/0000-0003-0910-8504)
Institutions
- Hauptman-Woodward Medical Research Institute (US)
- Brookhaven National Laboratory (US)
- University at Buffalo, State University of New York (US)
Publication Details
- Journal
- Acta Crystallographica Section F Structural Biology Communications
- Published
- 2025-05-29
- DOI
- https://doi.org/10.1107/s2053230x25004650
- Citations
- 2
- Primary Topic
- Enzyme Structure and Function
- Type
- article
- Field-Weighted Citation Impact
- 1.04
Funders
- U.S. Department of Energy
- National Institute of General Medical Sciences