Inactivation of Bioaerosol Particles in a Single-Pass Multi-Stage Non-Thermal Plasma and Ionization Air Cleaner

Bioaerosol particles contribute to the reduced indoor air quality and cause various health issues, thus their concentration must be managed. Air cleaning is one of the most viable technological options for reducing quantities of indoor air contaminants. This study assesses the effectiveness of a prototype multi-stage air cleaner in reducing bioaerosol particle viability and concentrations. The single-pass type unit consisted of non-thermal plasma (NTP), ultraviolet-C (UV-C) irradiation, bipolar ionization (BI), and electrostatic precipitation (ESP) stages. The device was tested under controlled laboratory conditions using Escherichia coli (Gram-negative) and Lactobacillus casei (Gram-positive) bacteria aerosol at varying airflow rates (50–600 m3/h). The device achieved over 99% inactivation efficiency for both bacterial strains at the lowest airflow rate (50 m3/h). Efficiency declined with increasing airflow rates but remained above 94% at the highest flow rate (600 m3/h). Among the individual stages, NTP demonstrated the highest standalone inactivation efficiency, followed by UV-C and BI. The ESP stage effectively captured inactivated bioaerosol particles, preventing re-emission, while an integrated ozone decomposition unit maintained ozone concentrations below safety thresholds. These findings show the potential of multi-stage air cleaning technology for reducing bioaerosol contamination in indoor environments, with applications in healthcare, public spaces, and residential settings.