Pushing the Boundaries of Resolution in Solid-State Nuclear Magnetic Resonance of Biomolecules with 160 kHz Magic-Angle Spinning
We present ultrafast magic-angle spinning (MAS) at 160 kHz in solid-state nuclear magnetic resonance (NMR), demonstrating unprecedented spectral quality and coherence lifetimes in proton-detected experiments on biomolecular systems. By optimizing experimental and sample conditions and utilizing advanced filling and handling tools, we achieve superior resolution on both microcrystalline and membrane-reconstituted proteins, paving the way for new applications in structural biology.
Authors
- Tanguy Le Marchand (ORCID: https://orcid.org/0000-0002-0814-1217)
- Armin Purea
- Claire Ollier
- Z. Sun (ORCID: https://orcid.org/0000-0002-7667-3178)
- Batiste Thienpont
- James N. Sturgis (ORCID: https://orcid.org/0000-0001-5125-7699)
- Kristof Grohe (ORCID: https://orcid.org/0000-0002-3388-4741)
- Guido Pintacuda (ORCID: https://orcid.org/0000-0001-7757-2144)
- Tatyana Polenova (ORCID: https://orcid.org/0000-0002-0346-1131)
- Lukas Becker (ORCID: https://orcid.org/0000-0003-1999-5741)
- Frank Engelke (ORCID: https://orcid.org/0000-0002-6296-7052)
- Sebastian Wegner
- Adrienn Rancz
Institutions
- Université Claude Bernard Lyon 1 (FR)
- École Normale Supérieure de Lyon (FR)
- Centre National de la Recherche Scientifique (FR)
- Bruker (United States) (US)
- Aix-Marseille Université (FR)
- University of Delaware (US)
Publication Details
- Journal
- Journal of the American Chemical Society
- Published
- 2025-06-02
- DOI
- https://doi.org/10.1021/jacs.5c02466
- Citations
- 6
- Primary Topic
- Advanced NMR Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 4.54
Funders
- Agence Nationale de la Recherche
- Centre National de la Recherche Scientifique
- H2020 European Research Council