IN VITRO ASSESSMENT OF THE BIOSORPTION POTENTIAL OF SOME REPRESENTATIVES OF ТНЕ GENUS BACILLUS DURING INTERACTION WITH LEAD CATIONS

The article presents studies on the detoxification of lead by bacteria of the genus Bacillus.In the experimental part.a combination of methods was used to fully assess the toxicity of lead cations and their effects on the growth of Bacillus bacteria in a model experiment.The data obtained indicate an inhibitory effect of Pb(NO3)2 and Pb(CH3COO)2 at concentrations from 1 mM to 0.063 mM on the test organisms.The presence of these cations in the nutrient substrate indicates the effect of lead on the growth of microorganisms.as the increase in optical density is due to their high sorption properties.Atomic absorption spectrophotometry and atomic force microscopy were used to study the biosorption of lead from the substrate.Assessment of detoxification.expressed in active accumulation of lead on microbial cell surfaces up to 65% during the stationary growth phase suggests the potential use of these microorganisms as microbial bio-remediators and bio-correctors to reduce excess lead content in bodies or ecosystems.