ISOLATION OF ETHOPROPHOS-DEGRADING BACTERIA AND PHYTOREMEDIATION–MICROBIAL REMEDIATION FROM SOIL FOR THE PROJECT OF WATER DIVERSION FROM THE YANGTZE RIVER TO CHAOHU LAKE

Ethoprophos contamination in soils in "the project of water diversion from the Yangtze River to Chaohu Lake" is a wide-ranging and non-negligible environmental problem.This study isolated ethoprophos-degrading microorganisms from typical riparian zones of the project area and conducted pot experiments using native herbaceous plants to evaluate the effectiveness of combined phytoremediation and microbial remediation for ethoprophos-contaminated soils.Five ethoprophos-degrading strains were successfully isolated and identified as Bacillus badius, Serratia liquefaciens, Klebsiella aerogenes, Stenotrophomonas pavanii, and Acinetobacter seifertii.While the degradation efficiencies of these strains decreased with increasing ethoprophos concentrations (from 10 mg/L to 50 mg/L), supplementation with 0.1 g/L glucose significantly enhanced their degradation capabilities.Among these strains, Acinetobacter seifertii demonstrated superior degradation efficiency, reaching a maximum of 80.75%, and was consequently selected for subsequent pot experiments.The combined remediation approach using Acinetobacter seifertii with Polygonum lapathifolium, Setaria viridis, and Carex dimorpholepis revealed that, after 30 days of treatment, the degradation efficiency of the combined system significantly outperformed individual microbial or plant treatments.Notably, the combination of Acinetobacter seifertii and Carex dimorpholepis achieved the highest degradation rate of 93.27%.These findings provide a promising bioremediation strategy for addressing ethoprophos contamination in soils.