Protein nanorotors control the size of lipid domains in phase-separated monolayers
Upon ATP addition, protein-enriched domains increased in size, while protein-devoid domains contracted. The observed changes followed the 2D Young-Laplace equation, where the spinning motion of ATP synthase reduces the lateral pressure in the protein-enriched phase. The unbalanced surface pressure between phases drives the domain size modulation; which is sensitive to variations in the surface pressure difference between lipid phases as small as 10<sup>-9</sup>N/m. These findings show that ATP synthase activity can dynamically regulate lipid phase separation by modifying interfacial properties and kinetic constraints.
Authors
- Iván López‐Montero (ORCID: https://orcid.org/0000-0001-8131-6063)
- Hernán A. Ritacco (ORCID: https://orcid.org/0000-0001-8878-4345)
- David Valdivieso González (ORCID: https://orcid.org/0000-0002-0511-9399)
- Paolo Natale (ORCID: https://orcid.org/0000-0002-4759-0816)
- Francisco J. Cao (ORCID: https://orcid.org/0000-0003-1611-913X)
- Víctor G. Almendro‐Vedia (ORCID: https://orcid.org/0000-0002-7297-1901)
- Nuria Carrillo-Godoy
Institutions
- Madrid Institute for Advanced Studies (ES)
- Universidad Complutense de Madrid (ES)
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Research Institute Hospital 12 de Octubre (ES)
- Instituto de Física del Sur (AR)
- Universidad Nacional del Sur (AR)
Publication Details
- Journal
- Journal of Colloid and Interface Science
- Published
- 2025-06-02
- DOI
- https://doi.org/10.1016/j.jcis.2025.138061
- Citations
- 1
- Primary Topic
- Lipid Membrane Structure and Behavior
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Banco Santander
- Ministerio de Ciencia, Innovación y Universidades
- Universidad Complutense de Madrid
- Consejo Nacional de Investigaciones Científicas y Técnicas
- Universidad Nacional del Sur
- Agencia Nacional de Promoción Científica y Tecnológica
- Fondo para la Investigación Científica y Tecnológica