The Effect of Different Media and BAP in Planlet Growth of <i>Phalaenopsis</i> OX Red Lion ‘The Conqueror’

Abstract Orchid is an ornamental plant that people love because it has flowers with beautiful shapes, colors, and patterns, and has high economic value. Orchids have very small seeds that are difficult to grow in natural conditions. One way to grow orchid seeds is by using tissue culture technique. Seeds of the hybrid orchid Phalaenopsis OX Red Lion ‘The Conqueror’ were cultured in an artificial medium and each medium has a different composition. Culture media can be added with growth regulators from cytokinins namely BAP that affect plant growth. The purpose of this study was to determine the effect of various media (MS, VW, Growmore) and BAP concentration (without BAP application, 0, 5, 1, and 1, 5 ppm) for the growth of hybrid orchid and the interaction between the two. Research data were analyzed by analysis of variance of 2 factors with 12 treatment combinations and 5 replications. If significantly different followed by Duncan’s Multiple Range Test (DMRT) at the 5% level. The results showed the combination of MS media and BAP 0, 5 ppm had the highest number of roots, while the BAP 0, 5 ppm produced the highest plantlet height and leaves length. Different media had an effect to planlet growth, MS media had the highest number of leaves, VW media had the highets number of shoots and root length, and Growmore media had the fastest root emergence time.