CRISPR/Cas9-mediated editing of VHL in induced pluripotent stem cells: A model for early cell fate in von Hippel-Lindau syndrome
The von Hippel-Lindau (VHL) tumor suppressor gene is crucial for cellular homeostasis, and its loss leads to VHL syndrome. To model early effects of VHL deficiency, we used CRISPR/Cas9 to generate human iPSC lines with heterozygous or homozygous out-of-frame deletions in exon 1. Both clones showed normal morphology, genomic stability, expression of undifferentiated markers, and tri-lineage differentiation potential. These models offer a valuable system to study early lineage specification and tumor initiation linked to VHL loss.
Authors
- Sven Gläsker (ORCID: https://orcid.org/0000-0003-2553-0086)
- Tim F. Grüner
- Aaron Thomas (ORCID: https://orcid.org/0000-0003-0477-5009)
- Jochen Dobner (ORCID: https://orcid.org/0000-0002-6336-9716)
- Haribaskar Ramachandran (ORCID: https://orcid.org/0009-0003-2481-675X)
- Andrea Rossi (ORCID: https://orcid.org/0000-0001-5863-6448)
- Barbara Hildebrandt
Institutions
- Vrije Universiteit Brussel (BE)
- Düsseldorf University Hospital (DE)
- Leibniz Institute of Environmental Medicine (DE)
- Heinrich Heine University Düsseldorf (DE)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2025-06-02
- DOI
- https://doi.org/10.1016/j.scr.2025.103748
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00