Isolated Escherichia coli Strain S591890 from a Wastewater Treatment Plant for Tetracycline Biodegradation

Tetracycline is a broad-spectrum antibiotic that is commonly used in both human and veterinary medicine. It is known to persist in the environment, including wastewater systems, where it can contribute to the development of antibiotic resistance in bacteria. This study aims to understand the mechanisms and potential of tetracycline removal through biodegradation by Escherichia coli strain S591890. The quantification of tetracycline was conducted using HPLC analysis. In addition, QTOF LC-MS was employed to investigate the biodegradation mechanisms of tetracycline. Analysis of tetracycline and its degradation products was conducted using liquid chromatography for separation and mass spectrometry for detection and identification. By monitoring the changes in the tetracycline peak and the appearance of degradation products over time, valuable insights into the degradation pathways and intermediates can be obtained. Furthermore, QTOF LC-MS enables the identification of specific metabolites formed during tetracycline biodegradation, providing valuable information about the transformation products and their potential toxicity.