Phytochemical Analysis of Polygonum bistorta L. Subsp. Carneum (Koch): Quantitative Analysis of Phenolics, Silver Nanoparticles Synthesis, and Biological Activity

Natural products are significant for food and pharmaceuticals due to their biological effects. The improvement of spectroscopy led to the isolation of bioactive compounds from plants and other organisms, and these compounds have been effectively used as raw pharmaceutical materials. Herein, bioactive compounds in Polygonum bistorta subsp. corneum aerial part was determined quantitatively by LC-MS/MS. Shikimic acid (mg/g extract) (2.85), catechin (1.88), chlorogenic acid (0.534), quercetin-3-D-xyloside (0.439), and hesperidin (0.355) were found as major products. The aerial part of this plant was employed for silver nanoparticles (AgNPs) synthesis. The aerial of the plant material was heated in deionized water for 2 hours, then filtrated. The filtrate was treated with AgNO3 (1.0 mM) to yield the AgNPs. Advanced spectroscopic and image techniques such as XRD, UV-Vis, Zetasizer, FTIR, and TEM were employed to identify the structure. The particle size of AgNPs was determined as 21.3 nm. DPPH assay and ABTS assay were exploited. AgNPs demonstrated better antioxidant activity than extract.