Study the effect of nanoparticle Probiotic from Enterococcus faecium as antibacterial and antibiofilm

Background: Enterococci, known for their importance effect, these bacteria are commonly found in food, environment, as well as the animal and human digestive systems. Nanoparticles (NPs) are increasingly used to target bacteria as an alternative to antibiotics. Probiotic bacteria were increase used as antimicrobial effect so this study were aimed to used the probiotic nanoparticle from strains of Enterococcus faecium as antibacterial and antibiofilm. Methods: Enterococcus were isolated from dairy product after biochemical characterization and vitek identification the isolate includes Enterococcus faecium. The isolate underwent for probiotic production and examined as antibacterial effect against E.coli, Pseudomonas aeruginosa, Staphylococcus and result declared the inhibitory effect against these bacteria. Results: The silver nanoparticles, or AgNPs, such as their optical, electrical, and antibacterial properties. AgNPs have significant antimicrobial activity, prevent resistance from developing, and have a broad synergistic effect when paired with other medications, making them viable candidates in the current antibiotic crisis, which is characterized by a rise in antimicrobial resistance (AMR) and a decline in newly made medications so the result declared their inhibitory effect against klebsiella and Enterococcus. Conclusion: Antibiofilm, on the other hand, were tested against both gram-positive and gram-negative bacteria, and result show more effect against gram positive than gram negative bacteria.