GC-MS Analysis and quantitative phytochemical profile of methanol extract of Pueraria tuberosa root tubers

AncientrnChinese, Indian, and African civilizations have all recorded the use of plantsrnby humans to treat a variety of illnesses. Conventional medical treatments arernstill used by a significant portion of the population. The therapeuticrnqualities of Pueraria tuberosa are widely recognized. The study aims to quantify thernphytoconstituents andrnto analyse the bioactive compounds from methanol extract of Pueraria tuberosa (Roxb. ex Willd.) DC tubers use Gas Chromatography and MassrnSpectrometry to ascertain medicinal properties of the plant. Quantitativernphytochemical analysis were done to quantify the phenols, flavonoids andrntannins in various alcoholic and aqueous extracts of P. tuberosa rootrntubers. The phytochemical constituents of methanol proportion of P.rntuberosa tubers were appraised by GC-MS (Gas Chromatography-MassrnSpectrometry) scrutiny to illustrate existence of phytochemical composites. Compounds had been identified by comparingrnextract's mass spectra fragmentation patterns along with retention guides tornthose found in published literature as well as computer library. Acetone extracts had therngreatest phenolic content (443.87mgGAE/g extract), then succeeded by methanolicrnextract (149.07mgGAE/g extract). Methanolic extract had highest flavonoidrnconcentration (673.76±43.64mg RE/g extract). Acetone extracts registered greaterrntannin content 290.41mg GAE/g extract subsequent to methanol extracts 92.03mgrnGAE/g extract. Methanol fraction analyzed by GC-MS revealed 29 distinctrnchemicals. 13-Docosenamide (Z) showed highest peak area of 9.81%, followed byrnSilane, 1, 2-ethanediylbis (trimethyl-CAS (9.66%), Hexadecanoic acid, methylrnester (9.52%) along with Octadecanoic acid methyl ester CAS (9.41%).The results of current investigationrnsubstantiate presence of several phytochemical elements in root tubers of P.tuberosa.rnCompounds that exist in methanolic fraction are found to possess antibacterial,rnantioxidant, and anticancer activities supporting the potential use of P.tuberosarnas a phytopharmaceutical source.