Immunostimulant Effect of Combination Extract of Garcinia mangostana, Moringa oleifera, and Phyllantus niruri based on Phagocytic Index and Titre Antibody Production in Mice

Single-form plants such as Garcinia mangostana, Moringa oleifera folium, and Phyllanthus niruri herb showed immunomodulatory activity. Sefa-Fit or combination extract (CE) is a herbal product that combines three plants (G. mangostana, M. oleifera, and P. niruri), expected to be used as an alternative therapy for immune system disorders. This study aimed to evaluate the in vivo immunostimulant efficacy of Sefa-Fit or combination extract (CE). The research used carbon clearance (for phagocytosis test), antibody titre production using the SRBC (sheep red blood cell) methods, and organ histopathology examination. The results showed that CE showed strong immunostimulant activity based on the phagocytic index parameter at doses of 195 and 390 mg/kg BW to be 1.55±0,08 and 1.51±0,05 (k>1.5). While the dose of 97.5 showed moderate immunostimulant activity (k=1.48). Based on antibody titre testing using the SRBC method, all three doses of CE showed higher antibody titres (1:512; 1:1054, and 1:512), respectively, than the negative control (1:256). The organ histopathology tests showed that damage to the spleen, liver, and kidneys was much less than the negative control (p<0.001). The CE contains secondary metabolites such as the flavonoid, alkaloid, terpenoid, and saponin groups. These findings showed that CE can be developed as an immunostimulant phytotherapy.