Morin Inhibits Inflammation, Oxidative Stress and Ferroptosis in Lipopolysaccharide-Induced Endometritis

Endometritis is a uterine condition that results in significant detriment to the advancement of the global dairy industry.Morin is a phyto-flavonoid compound known for its diverse biological functions.The effect of morin on LPS-triggered endometritis remained undetermined.In the current study, sixty adult mice were allocated to four (n = 15) treatment groups, i.e., Control, LPS, LPS + Morin, and Morin groups.Intrauterine infusion of LPS was used to establish an endometritis model in mice.An intraperitoneal injection of morin was administered to the mice 1 hour before an intrauterine infusion of LPS.After 24h of intrauterine injection of LPS, all the mice were euthanized.Our results revealed that morin rescued uterine injury by relieving the histopathological alterations caused by LPS.Morin significantly (P<0.05)reduced myeloperoxidase and pro-inflammatory cytokines following LPS exposure.It significantly (P<0.05)attenuated malondialdehyde production and iron accumulation while markedly enhanced glutathione and adenosine triphosphate levels in response to LPS exposure.Furthermore, morin treatment significantly increased mRNA and protein expression of TLR4.Moreover, it significantly (P<0.05)repressed TLR4 and down streamed NF-B signaling.Notably, morin (P<0.05)suppressed LPS-induced oxidative damage by scavenging ROS and enhancing antioxidant enzymes activity.Additionally, morin activated the Nrf2 pathway, which was downregulated by LPS, which enhanced the antioxidant defence system.Our findings demonstrated that morin confers protection against endometritis in mice by inhibiting inflammation, oxidative stress, and ferroptosis.