THE ROLE OF TRANSCRIPTION FACTOR NGALS IN THE DEVELOPMENT OF ARABIDOPSIS TAPROOT

To explore the NGAL1, NGAL2 and NGAL3 transcription factors of the RAV subfamily of the NGATHA family in Arabidopsis, and analyze the function of N gal -tri in Arabidopsis root development, from NGAL ngal1-2 ngal2-1 ngal3-1 genes.The loss-of -function triplet mutant Ngal-tri starts with an elongated taproot and develops earlier in the central cortex than the wide type.To investigate the function of NGAL1 in Arabidopsis root development, the wild-type Arabidopsis Columbia ecotype seeds were selected to construct a phylogenetic tree and analyze motifs to predict the gene function of the transcription factor NGAL family.Plants overexpressing Ngal1-p35s were significantly shorter than the wild type taproot, while the root length of the Ngal1 function loss mutant Ngal1-2 had no significant change compared with the wide type tap root, and the function loss triple mutant Ngal-Tri was significantly longer than the wide type.Analyzing the result, it may be due to the redundancy of Ngal1, Ngal2, and Ngal3 functions caused by the binary vector pCambia1300-pNgal1.NGAL1-GFP was transferred into N gal -Tri, resulting in a longer root system than wide type native roots.The transgenic plant Ngal-Tri p NGAL1:NGAL1-GFP obtained by transferring the binary vector p Cambia1300-p NGAL1:NGAL1-GFP into Ngal-Tri can better restore the abnormal root phenotype of the Ngal-Tri triple loss-of-function mutant, indicating the function of the Ngal family in root development.The design of Bi FC experiments revealed that Ngal1 physically interacts with TMO5 and LHW, respectively.In order to search for the genetic link between the Ngal family and TMO5, the study hybridized the functionally lost quadruple mutant TMO5 Ngal1 Tri.The results showed that under the Ngal Tri background, the loss of TMO5 function weakened Ngal to some extent, resulting in the root elongation and thickening phenotype of the triple function loss mutant.Based on the above research, it can be seen that NGALs transcription factors play a certain role in Arabidopsis root development, and this study will also have important reference value for plant root development and plant regulation research.