Effectiveness of treatment of bitches for infertility

The results of treatment of bitches for infertility of unknown etiology are presented in a comparative aspect. The main causes of infertility in bitches are considered. An analysis of biochemical indicators of blood serum before and after treatment by different methods was carried out. Histological changes in the ovaries under different treatment regimens of females were also compared and the effectiveness of treatment was established in terms of reproduction indicators (puppies after treatment was established). The aim of the research was to establish the effectiveness of different treatment regimens for bitches for infertility using the release hormone analogue Buserelin acetate and Hepatolic. The study involved 54 animals, among which 6 bitches were healthy (control group), the rest were infertile bitches, which were divided according to the principle of analogues into 3 experimental groups of 16 females each. Females of the first experimental group were administered Buserelin acetate (0.1 ml per 1 kg of body weight, intramuscularly, once). Females of the second experimental group were given Hepatolic in a dose of 0.1 ml/kg twice a day for 2 consecutive days. Animals of the third experimental group were administered Buserelin acetate and Hepatolic simultaneously. Blood serum was prepared using a standard method, and biochemical studies were performed using biochemical analyzers Sysmex XN-1000V™, China and ILab Taurus™, Italy. Histological preparations were prepared using standard methods (stained using the Elastica van Gieson staining kit (Merck, Germany). A significant increase in urea to 7.25 mmol/l, creatinine to 138.26 mmol/l, alkaline phosphatase to 134.28 mmol/l and ALT to 135.28 IU/l in infertile bitches was found. After treatment, in bitches of the 1st experimental group, a decrease in urea to 7.15 mmol/l was detected, in the 2nd to 5.36 mmol/l and in the 3rd to 4.22 mmol/l. Creatinine had a similar trend and its content in blood serum was 82.33 in the 1st experimental group, 75.29 in the 2nd and 68.29 in the 3rd, respectively. The content of alkaline phosphatase and ALT. At the same time, the level of calcium, phosphorus and chlorine tended to increase and after the treatment did not significantly differ from similar indicators in healthy bitches. Also, the restoration of the functional state of the ovaries was established in females of the 1st and 3rd experimental groups, which was characterized by the presence of follicles at the 2nd stage of development in histological preparations. During the studies, the best effectiveness of treatment was established in females of the 3rd experimental group, the fertilization rate of which was 93.75%, while in animals of the 1st experimental group it was 62.50 %, and in bitches of the 2nd experimental group it was 50.00 %.