The S100A10 Gene Expression in Oviduct Epithelial Cells of Iraqi Cows with Endometritis

The present study carried out to study the S100A10 gene Expression in Oviduct epithelial cells of Iraqi cows with endometritis in comparison with it in the oviduct epithelial cells of healthy Iraqi cows. Fifty uterine sample were collected from cows with macroscopic change in the uterus, the samples were taken from uterine endometrium and oviduct ampulla, samples were divided into two Into parts, one for molecular examination and second part for histopathological study. The histological examination of 50 endometrial samples of the cow’s reproductive tract revealed that 30 samples exhibited histological changes associated with endometritis, while the 20 samples were normal endometrium. The molecular study involved RNA extraction from oviduct tissue ampulla and RNA concentration measurement by Quantus™ Fluorometer , cDNA syntheses and Quantitative Reverse transcriptase PCR (RT-qPCR) Preparation, The gene expression analysis of S100A10 using RT-qPCR technology revealed a significant up-regulation in cows oviduct ampulla within endometritis compared to normal endometrium cows (p-value< 0.0001) the expression level (fold change) oviduct epithelial cell in endometritis cows was (2.346) whereas in oviduct epithelial cell of normal endometrium cows (1.038) ,In conclusion ,the result indicated that presence of gross lesion changes does not be necessarily indicate microscopic pathological change ,up-regulation in S100A10 in oviduct ampulla of cows with endometritis. Endometritis extends its effects on the oviduct by it is effect on the oviduct genes.