Preparation and Characterization of Ag, ZnO, and ZnO:Ag Nanoparticles Using the Pulsed Laser Ablation Method

Zinc oxide nanoparticles possess unique features enable them qualified to be applied in various fields such as medicine and the environment. In this project, ZnO, Ag and mixture (ZnO: Ag) nanoparticles were synthesized using The laser ablation (PLA) method which is one of the most popular and straightforward methods for generating these materials because it results in particles with high bioavailability and low toxicity. Various techniques were employed for analyzing and characterizing the nano-products. More, the x-ray data reflected that all the oxides have a polycrystalline cubic structure, with a preferred orientation along (111) for Ag NPs, and a preferred orientation along (100) for Zinc oxide, with a preferred orientation along for ZnO: Ag NPs (200). The produced nanoparticles were polydispersed, spherical in shape, densely scattered, and aggregated, according to the SEM micrographs of the particles. The uptake of Ag, ZnO, and ZnO: Ag NPs is significantly time-dependent and rather quick. A western blot was used to examine the impacts on the ability of skin cancer cells to migrate and invade, and the protein MMP that is connected to migration and invasion was examined. ZnO:Ag nanocomposites may offer a novel therapeutic approach for the targeted treatment of cancer cell. ZnO and ZnO: AgNPs uptake is strongly time-dependent and relatively rapid. There is a significant uptake within the first five hours for both ZnO and ZnOAg NPs. Thus, afterward, the skin cancer cell rate ingesting ZnO NPs and ZnOAg NPs was markedly reduced for five hours, reaching stable values at ten hours, indicating that the cell is saturated. ZnO: Ag NPs treatment resulted in lower protein levels than ZnONPs therapy. ZnO: Ag NPs are more effective than ZnO NPs at inhibiting migration and invasion in skin cancer cells. The reason may be that the adhesion of ZnO: Ag NPs on cell membrane is more than the penetration of it as conformed to cellular uptake results, which means that ability of ZnO: Ag NPs to interact with membrane proteins more than that of ZnO NPs.Keywords: Meat quality, Cerium oxide NPs, Zinc oxide NPs, Epididymal sperms, Ram.