Design, Synthesis, Characterization, and Evaluation of Anti-inflammatory Activity of Amino thiazole Coumarin Derivatives

This research aims to design, synthesize, and assess the in-vitro anti-inflammatory activity of aminothiazole coumarin derivatives by reacting 3-bromoacetyl coumarin with thiourea in ethanol to give 5 substituted coumarin derivatives. The synthesized compounds were designed & evaluated to investigate their binding interactions with the protein target 3PGH. All the newly synthesized aminothiazole coumarin derivatives are characterised by IR, 1H NMR and mass spectroscopy. Compounds were evaluated for in-vitro anti-inflammatory activity by using membrane stabilization assay & BSA denaturation assay, measuring their ability to modulate key markers of inflammation. Compounds 5b, 5c and 5e exhibited significant activity and Compound 5d showed moderate activity by membrane stabilization assay. Compound 5c exhibited significant activity & Compound 5c showed moderate activity by BSA denaturation assay. Compounds 5b and 5c are found to exert significant anti-inflammatory activity by membrane stabilization assay as well as BSA denaturation assay and can serve as potential compounds as anti-inflammatory agents to treat inflammation in future.