ANTICANCER AND ANTIOXIDANT ACTIVITIES OF PAPAINHYDROLYZED Α-LACTALBUMIN

In the present study, -lactalbumin (-La) was hydrolyzed with papain for different periods (30, 60, 90, and 120 min).The -La hydrolysate, which was obtained by subjecting papain to a hydrolysis process for 120 min, had the most significant antioxidant activity.The anticancer activity of these hydrolysates was evaluated on the Mcf-7, Caco-2, and A-549 human cancer cell lines using MTT assay, recording IC50 at 45.24, 79.9, and 49.17 g/mL, against Mcf-7, Caco2, and A-549, respectively.The expression levels of caspase-9 in Mcf-7, A-549, and Caco-2 cells treated with the IC50% (45.24, 49.17, and 79.9 g/mL -La hydrolysates) for 2 h increased by about 3.591, 2.609, and 3.195-fold, respectively, as compared to control.-La hydrolysates inhibited VEGFR-2 in and Caco2 cells by about 23,42,and 47%, respectively).The findings demonstrated that -La, hydrolyzed with papain, exhibited significant anticancer properties against human cancer cells.