Analysis of the Advantages and Disadvantages of PCR Technology and Ideas for Improvement

PCR began in the late 60s and early 70s of the 20th century for the study of gene in vitro isolation technology, in 1953 Watson and Crick proposed the double helix structure of DNA, in 1959 Kornberg reproduced the replication process of DNA in a test tube, in 1969 Taq enzyme was discovered, in 1984 PCR was proven to be feasible, since its inception, through many technical iterations, now in medicine, agriculture, Food safety testing and other fields are widely used as a mature technology, however PCR technology is not perfect, it cannot always provide satisfactory results in experiments, the exploration of PCR technology in improving specificity and accuracy is still a long way, the article introduces the development history of PCR, the mechanism, advantages and limitations of different PCR technologies, summarizes the technical points and precautions of traditional PCR through the analysis of operation methods and materials, and introduces the application of various PCR technologies. The development of PCR technology was also discussed. This article helps readers understand the existing problems of PCR and gives ideas for improvement, which is helpful for updating PCR technology.