Metformin inhibits the proliferation of hepatocellular carcinoma cells through inducing ferroptosis analyzed by phosphoproteomics
The results showed that ferrostatin-1 could partly reverse the inhibitory effects of Metformin, suggesting the involvement of ferroptosis. Moreover, the modulation of autophagy and ferroptosis-related proteins by Metformin was confirmed by western blot. Our findings demonstrate that Metformin induces ferroptosis in Bel-7402 and HepG2 cell lines, potentially offering a novel therapeutic strategy for the treatment of hepatocellular carcinoma. The phosphoproteomics analysis provides insights into the molecular mechanisms by which Metformin exerts its anti-cancer effects.
Authors
- Caide Lu (ORCID: https://orcid.org/0000-0001-9588-2218)
- Shuqi Mao (ORCID: https://orcid.org/0000-0003-0672-8126)
- Yuying Shan (ORCID: https://orcid.org/0000-0002-5168-3919)
- Jihan Sun (ORCID: https://orcid.org/0000-0002-3811-6434)
- Xin Yu (ORCID: https://orcid.org/0000-0003-0543-5567)
- Manyun Dai (ORCID: https://orcid.org/0000-0002-7184-5129)
- Andrea Gaucherot
Institutions
- Ningbo University (CN)
- Inserm (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- University of Clermont Auvergne (FR)
- Ningbo Medical Center Lihuili Hospital (CN)
- Ningbo College of Health Sciences (CN)
Publication Details
- Journal
- Frontiers in Oncology
- Published
- 2025-05-30
- DOI
- https://doi.org/10.3389/fonc.2025.1531420
- Primary Topic
- Ferroptosis and cancer prognosis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Natural Science Foundation of Ningbo