Optimization of Polygonatum Honey Pill Process Based on Polysaccharide Component Analysis

黄精作为药食同源的一类中药材，目前多数是经过“九蒸九晒”炮制去除刺激性后作为饮片使用，而由于黄精属于补益类药材，所以可以考虑是否能与蜂蜜结合制作蜜丸增强其药效。黄精当中化学成分多糖占比最大，而不同产地的黄精当中其黄精组成各不相同。因此首先对炮制后的黄精进行多糖的提取与分离，除去蛋白质后，使用柱前衍生化法对多糖当中的单糖组成进行分析，与标准品谱图进行对比，其主要由葡萄糖、半乳糖、甘露糖组成。其次根据不同影响因素设计正交实验与响应面实验，结合实验结果与响应面图进行分析，评价出制作工艺当中影响因素最大项与最佳制作工艺。Polygonatum, a traditional Chinese medicinal herb with food applications, is predominantly processed through the method of “nine steaming and nine drying” to eliminate its irritant properties and is commonly used as a medicinal slice. Given its classification as a tonic herb, the potential for enhancing its therapeutic efficacy by combining Polygonatum with honey to produce honey pills merits investigation. Among the chemical constituents of Polygonatum, polysaccharides are the predominant components, and their composition varies significantly depending on the geographical origin of the herb. To address this, the first step involves the extraction and purification of polysaccharides from processed Polygonatum. After protein removal, the monosaccharide composition of the polysaccharides is analyzed via pre-column derivatization, with results compared against standard chromatograms. The analysis confirms that the polysaccharides are primarily composed of glucose, galactose, and mannose. Subsequently, orthogonal experiments and response surface methodology are employed to evaluate the effects of key variables on the manufacturing process. By integrating experimental data with response surface plots, the most significant factors influencing the process and the optimal production parameters are identified systematically. This approach ensures a rigorous optimization of the protocol for developing Polygonatum-based honey pills, thereby enhancing their therapeutic efficacy while maintaining quality and consistency.