Soil-Gradient-Derived Bacterial Synthetic Communities Enhance Drought Tolerance in Quercus pubescens and Sorbus domestica Seedlings

Climate-change-induced drought threatens forest restoration by limiting seedling establishment. To address this, we developed synthetic bacterial communities (SynComs) tailored to support drought tolerance in two Mediterranean tree species, <i>Quercus pubescens</i> and <i>Sorbus domestica</i>. Bacteria were isolated from forest soil exposed to long-term drought, sampling across soil depths and root-associated compartments. We selected strains with key plant-beneficial traits, including exopolysaccharide (EPS) production, hormone synthesis (auxin, ABA), siderophore release, and osmotic tolerance. SynComs were assembled based on functional complementarity and ecological origin. Biofilm assays showed that even weak individual producers could enhance community-level performance. After initial screening on <i>Arabidopsis thaliana</i>, the most and least effective SynComs were tested on <i>Q. pubescens</i> and <i>S. domestica</i> seedlings. Compared to controls, the best-performing SynComs reduced the proportion of drought-symptomatic seedlings by 47% in <i>Q. pubescens</i> and 71% in <i>S. domestica</i>, outperforming single-strain inoculants. Notably, EPS-rich SynCom B aligned with the conservative root traits of <i>Q. pubescens</i>, while hormone-rich SynCom F matched the acquisitive strategy of <i>S. domestica</i>. Predictive modeling identified bacterial identity and symptom timing as key predictors of drought resilience. Our results highlight the value of matching microbial traits with plant strategies and drought context for climate-smart forest restoration.