Comamonas trifloxystrobinivorans sp. nov., encoding a novel esterase responsible for the degradation of trifloxystrobin

A Gram-stain-negative, strictly aerobic and motile bacterium capable of degrading trifloxystrobin, designated strain J-3^T, was isolated from activated sludge from Nanhu Wetland Park in Huaibei City, Anhui Province, PR China. Growth was observed at 0-1.0% NaCl (w/v; optimum: 0.5%) at 15-35 °C (optimum: 30 °C) and pH 5.0-8.0 (optimum: pH 7.5). Strain J-3^T could degrade 59.9% of 75.0 µM trifloxystrobin within 3 days of incubation. A novel esterase responsible for hydrolysing trifloxystrobin to trifloxystrobin acid, StrE, was identified in strain J-3^T; it exhibited low similarity (<i><</i>31%) with previously reported trifloxystrobin esterases, indicating its novelty. Phylogenetic analysis based on 16S rRNA gene sequences and genomes indicated that strain J-3^T was most closely related to strain <i>Comamonas odontotermitis</i> Dant 3-8^T. The 16S rRNA gene similarity between strain J-3^T and <i>C. odontotermitis</i> Dant 3-8^T was 97.7%. The average nucleotide identity and digital DNA-DNA hybridization values between strain J-3^T and strain <i>C. odontotermitis</i> Dant 3-8^T were 81.7% and 25.4%, respectively. The major fatty acids were C_16:0, summed feature 3 (C_16:1 <i> ω7</i>c and/or C_16:1 <i> ω6</i>c) and summed feature 8 (C_{18 : 1} <i> ω7</i>c and/or C_{18 : 1} <i> ω6</i>c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic analysis, strain J-3^T was considered to represent a novel species within the genus <i>Comamonas</i>, for which the name <i>Comamonas trifloxystrobinivorans</i> sp. nov. is proposed with strain J-3^T (=KCTC 8781^T=MCCC 1K09559^T) as the type strain.