Metagenomics or Metataxonomics: Best Practice Methods to Uncover the Sinus Microbiome
Long-read-metagenomic sequencing is the best method for analyzing the sinus microbiome. 16s-rRNA-sequencing (both long and short read) results in PCR amplification bias that significantly distorts the sinus microbiome.
Authors
- Alkis J. Psaltis (ORCID: https://orcid.org/0000-0003-2967-1855)
- Sarah Vreugde (ORCID: https://orcid.org/0000-0003-4719-9785)
- Emma F. Barry (ORCID: https://orcid.org/0000-0003-2742-5650)
- Kenny Yeo (ORCID: https://orcid.org/0000-0001-5733-0463)
- George Bouras (ORCID: https://orcid.org/0000-0002-5885-4186)
- Clare Cooksley (ORCID: https://orcid.org/0000-0003-3109-4540)
- Kevin Fenix (ORCID: https://orcid.org/0000-0003-1619-1406)
- James Connell (ORCID: https://orcid.org/0000-0002-3081-5433)
- Isabella Amy Burdon (ORCID: https://orcid.org/0000-0002-2464-7947)
- Peter J. Wormald
Institutions
- Basil Hetzel Institute (AU)
- Queen Elizabeth Hospital (AU)
- Central Adelaide Local Health Network
- The University of Adelaide (AU)
Publication Details
- Journal
- International Forum of Allergy & Rhinology
- Published
- 2025-05-30
- DOI
- https://doi.org/10.1002/alr.23617
- Citations
- 1
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- University of Adelaide
- Garnett Passe and Rodney Williams Memorial Foundation
- National Health and Medical Research Council