Novel CF₃-Substituted Pyridine- and Pyrimidine-Based Fluorescent Probes for Lipid Droplet Bioimaging
We have designed novel push-pull systems based on CF<sub>3</sub>-substituted pyridines and pyrimidines. The photophysical properties of these new fluorophores have been examined using both absorption and emission spectral analyses in acetonitrile solutions and solid states. All fluorophores proved to exhibit moderate absolute quantum yields of up to 0.33 in solutions and up to 0.12 in solid states, depending on their specific structures. Most fluorophores have demonstrated significant aggregation-induced emission behavior, making them suitable as robust and low-toxicity bioimaging agents for bioimaging studies. Comparison with known dyes and studies on various cell cultures demonstrated the selectivity of the obtained push-pull systems for visualizing lipid droplets.
Authors
- Valery N. Charushin (ORCID: https://orcid.org/0000-0002-9140-358X)
- D.L. Chizhov (ORCID: https://orcid.org/0000-0002-2877-6148)
- Gennady L. Rusinov (ORCID: https://orcid.org/0000-0001-8567-9435)
- Ekaterina F. Zhilina (ORCID: https://orcid.org/0000-0002-1657-440X)
- Артем С. Минин (ORCID: https://orcid.org/0000-0003-3305-3195)
- Yuriy A. Kvashnin (ORCID: https://orcid.org/0000-0002-1093-024X)
- Ekaterina M. Dinastiia (ORCID: https://orcid.org/0000-0002-0872-8851)
- Nadezhda S. Demina (ORCID: https://orcid.org/0000-0002-0884-8183)
- Egor V. Verbitskiy (ORCID: https://orcid.org/0000-0002-1482-206X)
- Natalia A. Verbitskaia
Institutions
- Ural Federal University (RU)
- Institute of Organic Synthesis (RU)
- Ural Branch of the Russian Academy of Sciences (RU)
Publication Details
- Journal
- International Journal of Molecular Sciences
- Published
- 2025-05-30
- DOI
- https://doi.org/10.3390/ijms26115271
- Primary Topic
- Lipid metabolism and biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministry of Education and Science of the Russian Federation
- Ural Branch, Russian Academy of Sciences
- Russian Academy of Sciences
- Postovsky Institute of Organic Synthesis