Novel Multilayer Polymeric Inserts of Doxycycline for Treatment of Periodontal Diseases

Objective: The objectives of this research was to formulate and evaluate novel multilayer chitosan inserts containing doxycycline hyclate for long term usage in periodontal diseases locally.  Methods: This research was carried out to formulate three different polymer based ( polymer used was chitostan) inserts that were uni, bi- and triple-layer inserts, and doxycycline hyclate as anti-microbial agents by means of solvent-casting method. The different layers of doxycycline loaded chitosan based inserts were tesed byFT- IR and DSC to evaluate the compatibilities of doxycycline hyclate and the chitosan and other additive present in this prepation. After compatibility study the physical parameters were examined. The physical properties included were difference in weight, thickness, durability, weight uniformity, % moisture absorbance, % moisture loss, swelling index, tensile strength, drug content uniformity, release pattern in-vitro antibacterial studies and stability and many more.  Results and Discussion: The evaluated data of various physicochemical properties indicates that all the inserts had same amount of drug as per content uniformity test and exhibited more than 150 times endurance capability. The percentage moisture loss varied between 24.155 ± 7.35 and 40.43 ± 11.737. Tensile strength was found to be more in case of bi and triple layer of inserts as compared to single layer, it varied in the range 1.55 0.0545to 4.543 ± 0.201 kg/mm2 . The drug pattern showed that the triple layer periodontal inserts exhibited controlled release drug pattern as compared to uni, bi layer inserts. The uni layer inserts exhibited the fasted release drug delivery pattern. The tripled layered drug loaded inserts continued their drug release for upto 17 days. The stability studies showed no signs of degradation, the degradation of the inserts were much less than the plain drug.  Conclusion: The study indicates that physicochemical parameters shown by all the inserts were found satisfactory. Thus, the inserts prepared as tri-layer could showed better performences and keep an efficient amount of drug for the treatment of Periodontitis.