Evaluation of Antimicrobial Efficacy of Different Endodontic Sealers Against Root Canal Microorganisms: An In Vitro Study

Objective: The present study aimed to evaluate the antimicrobial efficacy of endodontic sealers that are -MTA Fillapex, AH26, Apexit Plus, Zinc Oxide Eugenol (ZOE), and Zinc oxide (ZO) + Thyme oil against E. coli, P. aeruginouosa, S. aureus, and E. faecalis. Materials and Methods:The antimicrobial tests were performed using the Agar diffusion Method.Microorganisms were inoculated with Muller Hinton Agar, and Five holes were punched and filled with respective sealers: MTA Fillapex (Group 1); AH26 (Group2); Apexit Plus (Group 3); ZOE (Group 4) and ZO+ Thyme oil (Group 5).These plates were then incubated, zone of inhibition was checked after 24 hours.The data was statistically analyzed using one-way ANOVA and Tukey's Test (p<0.05).Results: All the sealers showed some level of antimicrobial efficacy against test microorganisms with the highest antimicrobial activity exhibited by Zinc oxide + Thyme oil followed by AH26, MTA Fillapex, Apexit Plus, and least by ZOE. Conclusion:Zinc oxide + Thyme oil exhibited the highest antimicrobial efficacy against all test microorganisms.