Marine microbial mock communities for validating rRNA gene amplicon sequencing

Mock communities are defined mixtures of cells or DNA for methods development, testing, or validation. Here, we present mock communities based on previously used 16S and 18S rRNA gene sequences from diverse marine microorganisms. Their use as controls for amplicon sequencing enables the detection of biased or aberrant runs.

Authors

Ariel Rabines (ORCID: https://orcid.org/0000-0003-3238-9033)
Andrew E. Allen (ORCID: https://orcid.org/0000-0001-5911-6081)
Robert H. Lampe (ORCID: https://orcid.org/0000-0002-0578-3935)
Hong Zheng (ORCID: https://orcid.org/0000-0003-0972-880X)
Bryce A. Ellman

Institutions

J. Craig Venter Institute (US)
Scripps Institution of Oceanography (US)
University of California San Diego (US)

Publication Details

Journal: Microbiology Resource Announcements
Published: 2025-05-30
DOI: https://doi.org/10.1128/mra.00298-25
Citations: 2
Primary Topic: Microbial Community Ecology and Physiology
Type: article
Field-Weighted Citation Impact: 3.43

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article

Marine microbial mock communities for validating rRNA gene amplicon sequencing

Ariel Rabines, Andrew E. Allen, Robert H. Lampe, Hong Zheng et al.

May 30, 2025

2 citations

Microbiology Resource Announcements

Microbial Community Ecology and Physiology

3.43

article

Marine microbial mock communities for validating rRNA gene amplicon sequencing

Ariel Rabines, Andrew E. Allen, Robert H. Lampe, Hong Zheng, Bryce A. Ellman

May 30, 2025article en

2 citations

Abstract

Mock communities are defined mixtures of cells or DNA for methods development, testing, or validation. Here, we present mock communities based on previously used 16S and 18S rRNA gene sequences from diverse marine microorganisms. Their use as controls for amplicon sequencing enables the detection of biased or aberrant runs.

Microbiology Resource AnnouncementsVol. 14(7)

J. Craig Venter Institute (US), Scripps Institution of Oceanography (US), University of California San Diego (US)

Life below water

Openalex Percentile: Top 9%

Microbial Community Ecology and Physiology

3.43

DOI Open Access PDF OpenAlex

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Marine microbial mock communities for validating rRNA gene amplicon sequencing — Ariel Rabines, Andrew E. Allen, et al. · Microbiology Resource Announcements (2025) | TGRS Research Map | TGRS