Mangiferin Reduces Degeneration in Swine Preantral Follicles Enclosed in Ovarian Tissue Cultured In Vitro in the Presence of Doxorubicin

This study assessed the potential protective effects of Mangiferin (MANGI) at various concentrations (10, 50 and 100 μM) on follicular survival and activation, enzyme activity of catalase (CAT) and superoxide dismutase (SOD), as well as the relative mRNA expression for BAX, BCL2 and SOD in porcine ovarian tissue cultured in vitro for 48 h with and without chemical stress induced by 0.3 μg/mL doxorubicin (DOX). The results indicated that when MANGI was associated with DOX, regardless of the tested concentration, MANGI increased (p < 0.05) follicle survival when compared to DOX alone, with only MANGI 10 + DOX and MANGI 50 + DOX treatments reducing follicular degeneration to levels comparable with the culture control. Regarding antioxidant enzymes, all treated groups decreased (p < 0.05) SOD activity compared to cultured control, while MANGI 10 treatment showed lower (p < 0.05) CAT activity compared to DOX treatment. Compared to cultured control, the mRNA levels of SOD were reduced (p < 0.05) in the MANGI 100, MANGI 10 + DOX and MANGI 100 + DOX treatments. In conclusion, supplementing the culture medium with MANGI attenuated the toxicity induced by DOX during the in vitro culture of pig preantral follicles enclosed in ovarian tissue.