Biochemical characterization of <i>Bifidobacterium bifidum</i> peptidoglycan <scp>d</scp>,<scp>l</scp>-endopeptidase BbMep that generates NOD2 ligands
Soluble peptidoglycan fragments produced by the gut bacteria are key effectors in microbiota-host crosstalk. Here, we biochemically characterized BbMep, an NlpC/p60 domain-containing peptidoglycan d,l-endopeptidase from <i>Bifidobacterium bifidum</i>, which efficiently digests Lys- or Orn-type sacculi. Digestion of human stool-derived muropeptides by BbMep enhances NOD2 activation.
Authors
- Shiliu Feng (ORCID: https://orcid.org/0009-0000-6062-6273)
- Jeric Mun Chung Kwan (ORCID: https://orcid.org/0000-0002-1310-5464)
- Yuan Qiao (ORCID: https://orcid.org/0000-0003-0188-0700)
- Evan Wei Long Ng (ORCID: https://orcid.org/0009-0001-7229-3792)
Institutions
- Nanyang Technological University (SG)
Publication Details
- Journal
- RSC Chemical Biology
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cb00086f
- Citations
- 1
- Primary Topic
- Microbial Metabolites in Food Biotechnology
- Type
- article
- Field-Weighted Citation Impact
- 1.12
Funders
- National Research Foundation
- National Research Foundation Singapore
- Ministry of Education - Singapore
- Nanyang Technological University
- Ministry of Education, India
- Bộ Giáo dục và Ðào tạo