Determination of 20 fluoroquinolones in milk based on magnetic molecularly imprinted microsphere and aggregation induced emission luminongen

The residues of fluoroquinolones in foods of animal origin posed many threats to the consumer's health. The aim of this study was to developed a magnetic molecularly imprinted microsphere and aggregation induced emission luminogen based semi-homogeneous method for determination of fluoroquinolones in milk. Results showed the limits of detection for determination of 20 fluoroquinolones in milk were in the range of 0.4-11 pg/mL. The sensitivities were increased for up to 4329 folds in comparison with fluorescein isothiocyanate labeled tracer. Due to the ultra-high sensitivity, the milk sample could be analyzed directly after a 100-fold dilution, and the recoveries from fluoroquinolones fortified blank milk samples were in the range of 69.68 %-97.19 %. The method performances were better than the previous fluorescent (immune)assays for fluoroquinolones. This is the first paper reporting the use of magnetic molecularly imprinted microsphere and aggregation induced emission luminogen for determination of veterinary drug in food sample.