Longitudinal Two-Photon Imaging of Fluorescently Labeled Neurons in a Live Mouse Hippocampus

Source: Ulivi, A. F., et. al. Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice. J. Vis. Exp. (2019) This video demonstrates longitudinal two-photon imaging of fluorescently-labeled pyramidal neurons in a live mouse, showcasing the preparation, cannula alignment, and imaging of dendrites and dendritic spines to study neuronal structure in the hippocampal CA1 region.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-05-29
DOI
https://doi.org/10.3791/23451
Primary Topic
Neuroinflammation and Neurodegeneration Mechanisms
Type
article
Field-Weighted Citation Impact
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article

Longitudinal Two-Photon Imaging of Fluorescently Labeled Neurons in a Live Mouse Hippocampus

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Journal of Visualized Experiments
Neuroinflammation and Neurodegeneration Mechanisms
article

Longitudinal Two-Photon Imaging of Fluorescently Labeled Neurons in a Live Mouse Hippocampus

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Abstract

Source: Ulivi, A. F., et. al. Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice. J. Vis. Exp. (2019) This video demonstrates longitudinal two-photon imaging of fluorescently-labeled pyramidal neurons in a live mouse, showcasing the preparation, cannula alignment, and imaging of dendrites and dendritic spines to study neuronal structure in the hippocampal CA1 region.

Journal of Visualized Experiments
Openalex Percentile: Top 84%
Neuroinflammation and Neurodegeneration Mechanisms
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