Imaging of Fluorescently-Labeled Motor Neurons in an Adult <i>Drosophila</i> Leg

Source: Guan, W., et. al. Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle. J. Vis. Exp. (2018) This video demonstrates imaging of Drosophila legs with green fluorescent protein-expressing motor neurons using confocal microscopy. A laser and two detectors capture GFP and cuticle autofluorescence. Images are processed by splitting channels, subtracting autofluorescence, and merging into a colored composite image to visualize axons within the leg segment.