Imaging of Neuronal Mitochondria Using a Serial Block-Face Scanning Electron Microscope

Source: Mukherjee, K., et. al. Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy. J. Vis. Exp. (2016)This video demonstrates the preparation, imaging, and analysis of mouse brain tissue using a serial block-face scanning electron microscope (SBFSEM) to visualize mitochondrial ultrastructure, enabling precise mapping of their morphology and spatial distribution in axonal and dendritic neuronal compartments.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-05-29
DOI
https://doi.org/10.3791/23518
Primary Topic
Advanced Electron Microscopy Techniques and Applications
Type
article
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article

Imaging of Neuronal Mitochondria Using a Serial Block-Face Scanning Electron Microscope

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Journal of Visualized Experiments
Advanced Electron Microscopy Techniques and Applications
article

Imaging of Neuronal Mitochondria Using a Serial Block-Face Scanning Electron Microscope

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article en

Abstract

Source: Mukherjee, K., et. al. Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy. J. Vis. Exp. (2016)This video demonstrates the preparation, imaging, and analysis of mouse brain tissue using a serial block-face scanning electron microscope (SBFSEM) to visualize mitochondrial ultrastructure, enabling precise mapping of their morphology and spatial distribution in axonal and dendritic neuronal compartments.

Journal of Visualized Experiments
Openalex Percentile: Top 95%
Advanced Electron Microscopy Techniques and Applications
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