Confocal Microscopy Imaging of Intact Dendritic Arbors and Spine Morphology in a Cleared Mouse Brain

Source: Pekarek, B. T., et. al., Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing. J. Vis. Exp. (2021)In this video, we demonstrate the preparation, imaging, and analysis of a cleared mouse brain to examine dendritic arbors, spine morphology, and neuronal distribution using confocal microscopy and 3D reconstruction techniques.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-05-29
DOI
https://doi.org/10.3791/23444
Primary Topic
Cell Image Analysis Techniques
Type
article
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article

Confocal Microscopy Imaging of Intact Dendritic Arbors and Spine Morphology in a Cleared Mouse Brain

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Journal of Visualized Experiments
Cell Image Analysis Techniques
article

Confocal Microscopy Imaging of Intact Dendritic Arbors and Spine Morphology in a Cleared Mouse Brain

TODO TODO
article en

Abstract

Source: Pekarek, B. T., et. al., Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing. J. Vis. Exp. (2021)In this video, we demonstrate the preparation, imaging, and analysis of a cleared mouse brain to examine dendritic arbors, spine morphology, and neuronal distribution using confocal microscopy and 3D reconstruction techniques.

Journal of Visualized Experiments
Openalex Percentile: Top 91%
Cell Image Analysis Techniques
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Confocal Microscopy Imaging of Intact Dendritic Arbors and Spine Morphology in a Cleared Mouse Brain — TODO TODO · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS