Selective Single-Neuron Laser Ablation in a Zebrafish Larva Using a Confocal Microscope

Source: Morsch, M.,  et. al., Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy. J. Vis. Exp. (2017) In this video, we explore the technique of high-intensity UV laser ablation for precisely targeting and studying individual neurons in transgenic zebrafish. We demonstrate how to prepare, immobilize, and visualize neuronal responses, highlighting the cellular dynamics of injury and recovery.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-05-29
DOI
https://doi.org/10.3791/23505
Primary Topic
Spectroscopy Techniques in Biomedical and Chemical Research
Type
article
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article

Selective Single-Neuron Laser Ablation in a Zebrafish Larva Using a Confocal Microscope

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Journal of Visualized Experiments
Spectroscopy Techniques in Biomedical and Chemical Research
article

Selective Single-Neuron Laser Ablation in a Zebrafish Larva Using a Confocal Microscope

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article en

Abstract

Source: Morsch, M.,  et. al., Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy. J. Vis. Exp. (2017) In this video, we explore the technique of high-intensity UV laser ablation for precisely targeting and studying individual neurons in transgenic zebrafish. We demonstrate how to prepare, immobilize, and visualize neuronal responses, highlighting the cellular dynamics of injury and recovery.

Journal of Visualized Experiments
Openalex Percentile: Top 91%
Spectroscopy Techniques in Biomedical and Chemical Research
DOISourceOpenAlex
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