Visualization of Calcium Influx in Ventral Midbrain Neurons Derived from Mouse Embryos

Source: Bancroft, E. A., et al. Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons. J. Vis. Exp. (2020) This video demonstrates the real-time visualization of calcium influx in ventral midbrain neurons derived from mouse embryos. Using a viral vector to express a calcium indicator, the neurons are imaged under a confocal microscope to track changes in fluorescence intensity during spontaneous and neurotransmitter-induced calcium activity.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2025-05-29
DOI
https://doi.org/10.3791/23460
Primary Topic
Neuroscience and Neuropharmacology Research
Type
article
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article

Visualization of Calcium Influx in Ventral Midbrain Neurons Derived from Mouse Embryos

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Journal of Visualized Experiments
Neuroscience and Neuropharmacology Research
article

Visualization of Calcium Influx in Ventral Midbrain Neurons Derived from Mouse Embryos

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Abstract

Source: Bancroft, E. A., et al. Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons. J. Vis. Exp. (2020) This video demonstrates the real-time visualization of calcium influx in ventral midbrain neurons derived from mouse embryos. Using a viral vector to express a calcium indicator, the neurons are imaged under a confocal microscope to track changes in fluorescence intensity during spontaneous and neurotransmitter-induced calcium activity.

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