Two-Photon Microscopy for Imaging Neuronal Morphology in a Mouse Pup

Source: Mizuno, H., et al. In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice. J. Vis. Exp. (2018)This video showcases two-photon microscopy being used to image neurons in the brain of an anesthetized transgenic mouse pup. The two-photon laser allows for deeper imaging with high spatial resolution and minimal light scattering, enabling the detailed capture of neuronal morphology, including dendritic projections.

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Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-05-29
DOI: https://doi.org/10.3791/23541
Primary Topic: Neuroscience and Neuropharmacology Research
Type: article
Field-Weighted Citation Impact: 0.00

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article

Two-Photon Microscopy for Imaging Neuronal Morphology in a Mouse Pup

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May 29, 2025

Journal of Visualized Experiments

Neuroscience and Neuropharmacology Research

article

Two-Photon Microscopy for Imaging Neuronal Morphology in a Mouse Pup

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May 29, 2025article en

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Journal of Visualized Experiments

Openalex Percentile: Top 87%

Neuroscience and Neuropharmacology Research

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