Detecting the Role of mi RNA Molecules and Methylation on Expression of BMAL1 Gene in Kids with Amyloid Leukemia in Mosul City

Leukaemia is the most common type of blood cancer among all ages marked by rapid growth of abnormal blood cells.Epigenetics entails the study of genomic modifications that do not change the core DNA nucleotide sequence.The study included 45 children aged 2 to 11years old referred to Al-Hadbaa Specialized Hospital between October and November 2024.The sample was divided into two groups: the first consisted of 35 children with leukaemia, and the second encompassed 10 samples as controls.Two millilitres of blood was obtained from the children, one ml was placed in an EDTA tube for DNA extraction and methylation assessmentand the second ml was placed in a Trizol tube for RNA extraction.The research comprises three phases.The initial task is to ascertain the gene expression level for the BAML1 genes.The second step is to measure the level of microRNA-155, which affects how genes are expressed.The third step is to determine the percentage of DNA methylation on the gene promoters using q-PCR.The study showed an elevation in gene expression levels of miRNA 155, recorded at 18.2 for leukaemia patients in compared to the control group.The study also showed a reduction in the expression level of the BMAL gene in leukaemia patients (measured at 0.46) compared to the control group.