Molecular detection and identification of Trichobilharzia: development of a LAMP, qPCR, and multiplex PCR toolkit
We provide a molecular toolkit consisting of LAMP, qPCR, and multiplex PCR. By exhibiting high sensitivity, Tricho-LAMP and Tricho-qPCR assays are potentially suitable for environmental DNA (eDNA)-based environmental monitoring of bird schistosomes, by both researchers and public health authorities. Multiplex PCR can be used for species determination without the need for further sequencing.
Authors
- Petr Horák (ORCID: https://orcid.org/0000-0001-9820-5044)
- Zikmund Bartoníček (ORCID: https://orcid.org/0000-0002-5093-8123)
- Tomáš Macháček (ORCID: https://orcid.org/0000-0002-6310-4099)
- Roman Leontovyč (ORCID: https://orcid.org/0000-0002-0035-949X)
- Jan Procházka
Institutions
- Charles University (CZ)
Publication Details
- Journal
- Parasites & Vectors
- Published
- 2025-05-30
- DOI
- https://doi.org/10.1186/s13071-025-06822-y
- Citations
- 4
- Primary Topic
- Parasite Biology and Host Interactions
- Type
- article
- Field-Weighted Citation Impact
- 6.87
Funders
- Univerzita Karlova v Praze
- Grantová Agentura České Republiky