The Effect of Quercetin Application on Desmin and Vimentin Levels in Ovariectomized Rats with Cyclophosphamide-Induced Cardiotoxicity

The aim of our study was to investigate the effect of quercetin on cyclophosphamideinduced cardiotoxicity.A total of 35 female rats were used in the study, divided into five groups of seven rats each.All of the rats, except those in the control group, underwent ovariectomy and had their ovaries removed.This eliminated the potential impact of hormones such as oestrogen and progesterone on the study.All rats were fed ad libitum.While the control and ovariectomy groups received no treatment, 50 mg/kg quercetin (oral) was administered to the quercetin group, 100 mg/kg cyclophosphamide (intraperitoneal) to the cyclophosphamide group, and 50 mg/kg quercetin (oral) and 100 mg/kg cyclophosphamide to the quercetin + cyclophosphamide group was applied.electrocardiography (ECG) measurements were taken before toxicity induction (day 0) and on day 5 after toxicity induction.At the end of the study, the rats were euthanized under anesthesia in accordance with ethical rules, and tissue and blood samples necessary for analysis were taken.Troponin, creatine kinase (CK), and creatinine kinase MB (CK-MB) parameters were measured in the blood samples taken.Histopathological and immunohistochemical analyses (desmin and vimentin) were performed on heart tissue.According to the analysis, an increase in troponin, CK, and CK-MB parameters was observed in the cyclophosphamide group, while a decrease was observed in the quercetin group.In desmin and vimentin immunoreactivity, a decrease was observed in the cyclophosphamide group, while an increase was observed in the quercetin group.In conclusion, in our study, we demonstrated that quercetin has a positive effect against cyclophosphamide-induced cardiotoxicity.