Development and validation of an innovative HPLC-UV method for determination of Metalaxyl-M in flowable suspension fungicide formulas

Metalaxyl-M is a modern and more efficient version of metalaxyl which is designated to provide effective control of damaging fungal diseases. This work aimed to develop and validate a low-cost, accurate, fast, and simple analytical method for determination of active ingredient metalaxyl-M using high-performance liquid chromatography with ultraviolet detection (HPLC-UV). The development of the novel method was performed on reversed-phase brownlee™ C-18 column at constant temperature 30 ºC. The mobile phase consists of acetonitrile and distilled water in volumetric ratio 62.5:37.5, a flow rate of 1.2 mL/min, and the detective wavelength at 270 nm. The validation protocol underscore in our study was SANCO/3030/99 rev. 5. Specificity, linearity, LOD, LOQ, precision, recovery, and accuracy parameters were calculated and discussed. The values for multiple correlation coefficient (R2 ≥ 0.990), relative standard deviation (RSD < 1.5 %), recoveries ranged from (99.21 - 101.16 %) were found within acceptance criteria.