Development of Psoriasis-Like 3D Skin Models Using the HaCaT Keratinocyte Cell line as Potential Tools in Psoriasis Research

This study focused on developing a psoriasis-like 3D skin model (RHE-Pso) utilizing HaCaT cells , which were treated with cytokine cocktails to serve as a platform for identifying anti-psoriasis agents.Reconstructed HacaT cells on culture with cytokine cocktails on day 11 of high calcium condition were inducedWe compared the morphology, epidermal protein, and cytokine release between the RHE-Pso and a control ( RHE) that was not exposed to cytokines.The RHE-Pso model was subjected to two different cytokine formulas, which are known to be associated with psoriasis.Our findings revealed that the F1 formula (F1: 30 ng/mL TNF-, 30 ng/mL IL-1 and 50 ng/mL IFN-) significantly altered the histological structure of the model more than the F2 formula (F2: 30 ng/mL IL-17 and 30 ng/mL IL-22), and resulted in lower filaggrin protein expression.Additionally, the RHE-Pso model induced with the F1 formula showed a markedly higher production of pro-inflammatory cytokines, including IL-1, IL-6, IL-8, IFN-, and TNF-.Treatment of the RHE-Pso model with clobetasol propionate, an anti-psoriasis medication, effectively reduced tissue damage and decreased levels of inflammatory cytokines compared to the untreated model.This study demonstrates the potential of the RHE-Pso model as a valuable tool for exploring and validating new therapeutic compounds for psoriasis.