Antinociceptive and Anti-urolithiatic Effects of Ensete glaucum (Roxb.) Cheesman Seed Aqueous Extract in Mice

Ensete glaucum (Roxb.)Cheesman (E.glaucum) seeds are commonly used in Vietnamese folk medicine to treat urinary stones, edema, and osteoarthritis-related problems.Nevertheless, no reported scientific evidence has been found to substantiate these traditional practices.This study focused on investigating the potential antinociceptive and anti-urolithiatic properties of E. glaucum seed aqueous extract (EGE).The antinociceptive effect of EGE was evaluated in mice using thermal (hot plate test) and chemical (acetic acid and formalin-induced nociception test) pain models at various doses (50, 100, 200, 400 mg/kg; p.o.).The anti-urolithiatic activity of the EGE (200, 400 mg/kg; p.o.) was assessed in the sodium glyoxylate-induced urolithiasis in mice and in vitro nucleation and aggregation assays.EGE had potential against urolithiasis through its ability to modify several serum and urine biochemical parameters on glyoxylate-induced nephrolithiasis.The extract at the dose of 400 mg/kg significantly improved the inflammatory cells, kidney tissue structure, and renal calcification.The extract also exhibited significant in vitro anti-urolithiatic, anti-inflammatory, and antioxidant activities.These outcomes suggest that E. glaucum aqueous seed extract possesses antinociceptive activity and may aid in preventing urinary stones.Further studies are needed to elucidate the effectiveness of E. glaucum seeds in the analgesic activity and management of urolithiasis disease.