Innovative nucleic acid detection of Clostridioides difficile utilizing the PAM-unconventional, one-step LAMP/CRISPR-Cas12b detection platforms
Our research presents innovative CRISPR-based one-step nucleic acid detection platforms that eliminate canonical PAM sequence requirements. These platforms exhibit high sensitivity and specificity while achieving rapid detection under simple conditions, making them promising candidates for clinical diagnostics and point-of-care testing (POCT).
Authors
- Yizhuo Zhang (ORCID: https://orcid.org/0000-0002-6128-3476)
- Xu Su (ORCID: https://orcid.org/0009-0001-7842-065X)
- Yijian Chen
- Ling Lv (ORCID: https://orcid.org/0000-0002-8893-3889)
Institutions
- Fudan University (CN)
- Huashan Hospital (CN)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2025-05-29
- DOI
- https://doi.org/10.3389/fcimb.2025.1594271
- Citations
- 2
- Primary Topic
- Clostridium difficile and Clostridium perfringens research
- Type
- article
- Field-Weighted Citation Impact
- 2.79