A robust in-vitro and ex-vitro Agrobacterium rhizogenes-mediated hairy root transformation system in mungbean for efficient visual screening of transformants using the RUBY reporter

We have developed a simple, rapid, low-cost, and labor-efficient Agrobacterium rhizogenes-mediated mungbean transformation protocol using both in-vitro and ex-vitro approaches, with RUBY as a reporter gene. This method enables the generation of composite mungbean plants that are easier to handle, exhibit higher transformation efficiency, and can be effectively used for root specific functional genomics studies. We expect this technology to be widely adopted for investigating root-related processes in mungbean and other plant species.